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This is a confocal laser scanning micrograph of a 3-D lung model formed in the RCCS, derived from the A549 lung epithelial cell line stained with an antibody against the tight junctional marker, beta catenin. Photo credit(s) – Aurélie Crabbé; Cheryl Nickerson


This is a confocal laser scanning micrograph of a 3-D lung model formed in the RCCS ,derived from the A549 lung epithelial cell line stained with an antibody against the mucin marker, MUC5AC. Photo credit(s) –Aurélie Crabbé; Cheryl Nickerson


This is a confocal laser scanning micrograph of a 3-D lung model formed in the RCCS, derived from the A549 lung epithelial cell line stained with an antibody against the mucin marker, MUC5AC. Photo credit(s) –Aurélie Crabbé; Cheryl Nickerson


3-D HT-29 colon model
Description - This is a scanning electron micrograph (350X) of a 3-D colon model formed in the Synthecon RCCS, derived from the HT-29 colonic epithelial cell line. Photo credit(s) – Mark Ott; Cheryl Nickerson

3D Cell Models for Tissue Engineering in the RCCS


Our RCCS bioreactor system has been used to form a variety of 3D cell models for various applications. 


Here are a few applications for this novel system:


Co-culture
Spheroid formation
Tissue engineering (with or without scaffolds)
Host-pathogen studies
Formation of vascaularized tissue 
Explant maintenance
Cell therapy and regenerative medicine
Stem cell expansion
Stem cell differentiation of cells
Modeling microgravity
Vascular graft culture
Single Use 3D BioreactorsAutoclavable 3D BioreactorsStem Cell Culture SystemsPerfusion Bioreactors Nanobiomatrix ScaffoldsBiostructure Matrix Scaffolds
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