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Research Publications / Stem Cells

A novel perfused rotary bioreactor for cardiomyogenesis of embryonic stem cells
Teo A, Mantalaris A, Song K, Lim M
Biotechnol Lett. 2014 May;36(5):947-60. doi:10.1007/s10529-014-1456-y.
Accelerated and Improved Differentiation of Retinal Organoids from Pluripotent Stem Cells in Rotating-Wall Vessel Bioreactors.
DiStefano T, Chen HY, Panebianco C, Kaya KD, Brooks MJ, Gieser L, Morgan NY, Pohida T, Swaroop A
Stem Cell Reports. 2018 Jan 9;10(1):300-313. doi: 10.1016/j.stemcr.2017.11.001.
Adrenomedullin is a key Protein Mediating Rotary Cell Culture System that Induces the Effects of Simulated Microgravity on Human Breast Cancer Cells
Li Chen, Xi Yang, Xiang Cui, Minmin Jiang, Yu Gui, Yanni Zhang, Xiangdong Luo
Microgravity Science and Technology November 2015, Volume 27, Issue 6, pp 417-426
Advanced 3D models culture to investigate mesenchymal stromal cells of the human dental follicle.
Steimberg N, Angiero F, Farronato D, Berenzi A, Cossellu G, Ottonello A, Kaigler D, Mazzoleni G
Tissue Eng Part C Methods. 2018 Jan 12. doi: 10.1089/ten.TEC.2017.0428
Aligned Nanofibrous Cell-Derived Extracellular Matrix for Anisotropic Vascular Graft Construction.
Xing Q, Qian Z, Tahtinen M, Yap AH, Yates K, Zhao F
Adv Healthc Mater. 2017 May;6(10). doi: 10.1002/adhm.201601333. Epub 2017 Feb 9
An integrated biomanufacturing platform for the large-scale expansion and neuronal differentiation of human pluripotent stem cell-derived neural progenitor cells.
Srinivasan G, Morgan D, Varun D, Brookhouser N, Brafman DA.
Acta Biomater. 2018 74:168-179
Beta-adrenoceptor pathway enhances mitochondrial function in human neural stem cells
Chiang MC, Lin H, Cheng YC, Yen CH, Huang RN, Lin KH
J Neurosci Methods. 2012 Jun 15
Billion-scale production of hepatocyte-like cells from human induced pluripotent stem cells.
Yamashita T, Takayama K, Sakurai F, Mizuguchi H.
Biochem Biophys Res Commun. 2018 496(4):1269-1275
Bioreactor Cultivation Enhances NTEB Formation and Differentiation of NTES Cells into Cardiomyocytes
Lu S, Liu S, He W, Duan C, Li Y, Liu Z, Hao T, Wang Y, Li D, Wang C, Gao S
Cloning Stem Cells 10:363-370, 2008
Bioreactor Cultivation Enhances the Efficiency of Human Embryoid Body (hEB) Formation and Differentiation
Gerecht-Nir S, Cohen S, Itskovitz-Eldor J
Biotechnology and Bioengineering 86: 493-502, 2004
Bioreactor Expansion of Human Bone Marrow Mesenchymal Stem Cells
Chen X, Xu H, Wan C, McCaigue M, Li G
Stem Cells 24: 2052-2059, 2006
Cell-cell and cell-extracellular matrix interactions regulate embryonic stem cell differentiation
Chen SS, Fitzgerald W, Zimmerberg J, Kleinman HK, Margolis L
Stem Cells 25: 553-561, 2007
Cementum protein 1 (CEMP1) induces differentiation by human periodontal ligament cells under three-dimensional culture conditions
Hoz L, Romo E, Zeichner-David M, Sanz M, Nuñez J, Gaitán L, Mercado G, Arzate H
Cell Biol Int.2012 Feb 1
Characteristics of mesenchymal stem cells derived from Wharton's jelly of human umbilical cord
Liu S, Hou KD, Yuan M, Peng J, Zhang L, Sui X, Zhao B, Xu W, Wang A, Lu S, Guo Q
J Biosci Bioeng. 2013 Jul 27
Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system
Chen L, Liu G, Li W, Wu X
Cell Mol Biol Lett. 2019; 24:16
Chondrogenic differentiation of human adipose derived stem cells using microcarrier and bioreactor combination technique
Kang H, Lu S, Peng J, Yang Q, Liu S, Zhang L, Huang J, Sui X, Zhao B, Wang A, Xu W, Guo Q, Song Q
Mol Med Rep. 2014 Oct 30. doi: 10.3892/mmr.2014.2820
Coculture of meniscus cells and mesenchymal stem cells in simulated microgravity.
Weiss WM, Mulet-Sierra A, Kunze M, Jomha NM, Adesida AB
NPJ Microgravity. 2017 Nov 10;3:28. doi: 10.1038/s41526-017-0032-x. eCollection 2017
Complex Extracellular Matrices Promote Tissue-specific Stem Cell Differentiation
Philp D, Chen SS, Fitzgerald W, Orenstein J, Margolis L, Kleinman HK
Stem Cells 23: 288-296, 2005
Creation of engineered cardiac tissue in vitro from mouse embryonic stem cells
Guo XM, Zhao YS, Chang HX, Wang CY, Ling-Ling E, Zhang XA, Duan CM, Dong LZ, Jiang H, Li J, Song Y, Yang XJ
Circulation 113 :2229-2237, 2006
Development of a Three-Dimensional Bioengineering Technology to Generate Lung Tissue for Personalized Disease Modeling
Wilkinson DC, Alva-Ornelas JA, Sucre JM, Vijayaraj P, Durra A, Richardson W, Jonas SJ, Paul MK, Karumbayaram S, Dunn B, Gomperts BN
Stem Cells Transl Med. 2016 Sep 15. pii: sctm.2016-0192
Differentiation of human mesenchymal stem cell spheroids under microgravity conditions
Cerwinka WH, Sharp SM, Boyan BD, Zhau HE, Chung LWK, Yates C
Cell Regeneration 2012
Differentiation of transforming growth factor β1-induced mesenchymal stem cells into nucleus pulposus-like cells under simulated microgravity conditions.
Han C, Jiang C, Yu C, Shen H.Cell
Mol Biol (Noisy-le-grand). 2015 May 16;61(2):50-5
Early exposure of murine embryonic stem cells to hematopoietic cytokines differentially directs definitive erythropoiesis and cardiomyogenesis in alginate hydrogel three-dimensional cultures
Fauzi I, Panoskaltsis N, Mantalaris A.
Stem Cells Dev. 2014 Jun 13. (Epub ahead of print)
Effect of dynamic three-dimensional culture on osteogenic potential of human periodontal ligament-derived mesenchymal stem cells entrapped in alginate microbeads
Vecchiatini R, Penolazzi L, Lambertini E, Angelozzi M, Morganti C, Mazzitelli S, Trombelli L, Nastruzzi C, Piva R
J Periodontal Res. 2014 Sep 23. doi: 10.1111/jre.12225
Effect of microgravity on the mesenchymal stem cell characteristics of limbal fibroblasts.
Pao SI, Chien KH, Lin HT, Tai MC, Chen JT, Liang CM
J Chin Med Assoc. 2017 May 18. pii: S1726-4901(17)30109-0. doi: 10.1016/j.jcma.2017.01.008.
Effects of chondrogenic and osteogenic regulatory factors on composite constructs grown using human mesenchymal stem cells, silk scaffolds and bioreactors
August A, Marolt D, Freed LE, Vepari C, Meinel L, Farley M, Fajardo R, Patel M, Gray M, Kaplan DL, Vunjak-Novakovic G
J R Soc Interface. 2008 Aug 6;5(25):929-39
Efficient large-scale generation of functional hepatocytes from mouse embryonic stem cells grown in a rotating bioreactor with exogenous growth factors and hormones
Zhang S, Zhang Y, Chen L, Liu T, Li Y, Wang Y, Geng Y
Stem Cell Res Ther. 2013 Dec 2;4(6):145
Embryonic-like stem cells from umbilical cord blood and potential for neural modeling
McGuckin C, Forraz N, Baradez MO, Basford C, Dickinson AM, Navran S, Hartgerink JD
Acta Neurobiol 66: 321-329, 2006
Engineered bone tissue associated with vascularization utilizing a rotating wall vessel bioreactor
Nishi M, Matsumoto R, Dong J, Uemura T
J Biomed Mater Res A. 2012 Aug 3
Engineering of Functional Cartilage Tissue Using Stem Cells from Synovial Lining: A Preliminary Study
Pei M, He F, Kish VL, Vunjak-Novakovic G
Clin Orthop Relat Res 466: 1880-1889, 2008
Engineering three dimensional micro nerve tissue using postnatal stem cells from human dental apical papilla
Kim BC, Jun SM, Kim SY, Kwon YD, Choe SC, Kim EC, Lee JH, Kim J, Suh JF, Hwang YS
Biotechnol Bioeng. 2016 Oct 24. doi: 10.1002/bit.26205
Exosomes derived from umbilical cord mesenchymal stem cells in mechanical environment show improved osteochondral activity via upregulation of LncRNA H19
Yan L, Liu G, and Wu X.
J Orthop Translat 26, 2021; 111–120
Freeze-dried human serum albumin improves the adherence and proliferation of mesenchymal stem cells on mineralized human bone allografts
Weszl M, Skaliczki G, Cselenyák A, Kiss L, Major T, Schandl K, Bognár E, Stadler G, Peterbauer A, Csönge L
J Orthop Res. 2012 Mar
Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor
Takebe T, Kobayashi S, Kan H, Suzuki H, Yabuki Y, Mizuno M, Adegawa T, Yoshioka T, Tanaka J,Maegawa J, Taniguchi H
Transplant Proc. 2012 May
Hypoxia Created Human Mesenchymal Stem Cell Sheet for Prevascularized 3D Tissue Construction
Zhang L, Xing Q, Qian Z, Tahtinen M, Zhang Z, Shearier E, Qi S, Zhao F
Adv Healthc Mater. 2015 Dec 14. doi: 10.1002/adhm.2
Improvement of culture conditions of human embryoid bodies using a controlled perfused and dialyzed bioreactor system
Come J, Nissan X, Aubry L, Tournois J, Girard M, Perrier AL, Peschanski M, Cailleret M
Tissue Eng Part C Methods 14: 289-298, 2008
Increased proliferation and adhesion properties of human dental pulp stem cells in PLGA scaffolds via simulated microgravity.
He L, Pan S, Li Y, Zhang W, Yi H, Song C, Niu Y
Int. Endod J. 2015 Feb 19. doi: 10:1111/iej.12441
Induction of mesenchymal stem cell chondrogenic differentiation and functional cartilage microtissue formation for in vivo cartilage regeneration by cartilage extracellular matrix-derived particles.
Yin H, Wang Y, Sun Z, Sun X, Xu Y, Li P, Meng H, Yu X, Xiao B, Fan T, Wang Y, Xu W, Wang A, Guo Q, Peng J, Lu S
Acta Biomater. 2016 Jan 20. pii: S1742-7061(16)30024-1. doi: 10.1016/j.actbio.2016.01.024
Integrated 3-Dimensional Expansion and Osteogenic Differentiation of Murine Embryonic Stem Cells
Randle WL, Cha JM, Hwang YS, Chan KL, Kazarian SG, Polak JM, Mantalaris A
Tissue Eng 13: 2957-2970, 2007
Low gravity rotational culture and the integration of immunomodulatory stem cells reduce human islet allo-reactivity.
Qureshi KM, Lee J, Paget MB, Bailey CJ, Curnow SJ, Murray HE, Downing R
Clin. Transplant. 2015 Jan;29(1):90:8. doi: 10.1111/ctr.12488. Epub 2014 Dec 5.
Mesenchymal stem cells support growth and organization of host-liver colorectal-tumor organoids and possibly resistance to chemotherapy.
Devarasetty M1, Wang E, Soker S, Skardal A.
Biofabrication. 2017 Jun 7;9(2):021002. doi: 10.1088/1758-5090/aa7484.
Modeled Microgravity Disrupts Collagen I/integrin Signaling During Osteoblastic Differentiation of Human Mesenchymal Stem Cells
Meyers VE, Zayzafoon M, Gonda SR, Gathings WE, McDonald JM
J Cell Biochem. 93: 697-707, 2004
Modeled Microgravity Inhibits Osteogenic Differentiation of Human Mesenchymal Stem Cells and Increases Adipogenesis
Zayzafoon M, Gathings WE, McDonald JM
Endocrinology: 145 2421-2432, 2004
NASA approved rotary bioreactor enhances proliferation and osteogenesis of human periodontal ligament stem cells
Li S, Ma Z, Niu Z, Qian H, Xuan D, Hou R, Ni L
Stem Cells Dev. 18:1273-82, 2009
Nano-ceramic composite scaffolds for bioreactor-based bone engineering
Lv Q, Deng M, Ulery BD, Nair LS, Laurencin CT
Clin Orthop Relat Res. 2013 Aug
Neural Stem Cell Differentiation in a Cell-collagen-bioreactor Culture System
Lin HJ, O’Shaughnessy TJ, Kelly J, Ma W
Dev Brain Res. 153: 163-173, 2004
Non-genetic direct reprogramming and biomimetic platforms in a preliminary study for adipose-derived stem cells into corneal endothelia-like cells
Dai Y, Guo Y, Wang C, Liu Q, Yang Y, Li S, Guo X, Lian R, Yu R, Liu H, Chen J
PLoS One. 2014 Oct 15;9(10):e109856. doi:10.1371/journal.pone.0109856
Novel Bioreactor Platform for Scalable Cardiomyogenic Differentiation from Pluripotent Stem Cell-Derived Embryoid Bodies
Rungarunlert S, Ferreira JN, Dinnyes A
Methods Mol Biol. 2016 Apr 5
Novel alginate 3D static and rotating culture systems for effective ex vivo amplification
Yuan Y, Sin WY, Xue B, Ke Y, Tse KT, Chen Z, Xie Y, Xie Y
Transfusion. 2013 Feb 5
Numerical Simulation of Mass Transfer and Three-Dimensional Fabrication of Tissue-Engineered Cartilages Based on Chitosan/Gelatin Hybrid Hydrogel Scaffold in a Rotating Bioreactor.
Zhu Y, Song K, Jiang S, Chen J, Tang L, Li S, Fan J, Wang Y, Zhao J, Liu T.
Appl Biochem Biotechnol. 2016 Aug 15.
One-step articular cartilage repair: combination of in situ bone marrow stem cells with cell-free poly(L-lactic-co-glycolic acid) scaffold in a rabbit model
Shi J, Zhang X, Zeng X, Zhu J, Pi Y, Zhou C, Ao Y
Orthopedics. 2012 May
Paclitaxel-releasing mesenchymal stromal cells inhibit the growth of multiple myeloma cells in a dynamic 3D culture system.
Bonomi A1, Steimberg N2, Benetti A, Berenzi A, Alessandri G, Pascucci L, Boniotti J, Coccè V, Sordi V, Pessina A, Mazzoleni G
Hematol Oncol. 2016 Jun 10. doi: 10.1002/hon.2306
Production of stem cells with embryonic characteristics from human umbilical cord blood
McGuckin CP, Forraz N, Baradez MO, Navran S, Zhao J, Urban R, Tilton R, Denner L
Cell Prolif 38: 245-255, 2005
Properties of mechano-transduction via simulated microgravity and its effects on intracellular trafficking of VEGFRs
Puca A, Russo G, Giordano A
Oncotarget. 2012 Apr
Reconstruction of Functional Cortical-Like Tissues from Neural Stem and Progenitor Cells
Ma W, Tavakoli T, Chen S, Maric D, Liu JL, O'Shaughnessy TJ
Tissue Eng Part A 14: 1687-1697, 2008
Rhythmicity of Engraftment and Altered Cell Cycle Kinetics of Cytokine-Cultured Murine Marrow in Simulated Microgravity Compared With Static Cultures
Colvin GA, Lambert JF, Carlson JE, McAuliffe CI, Abedi M, Quesenberry PJ
In Vitro Cell Dev Bio Anim 38: 343-351, 2002
Rotary Suspension Culture Enhances Mesendoderm Differentiation of Embryonic Stem Cells Through Modulation of Wnt/β-catenin Pathway
Lei X, Deng Z, Zhang H, Zhao H, Zhou J, Liu S, Chen Q, Ning L, Cao Y, Wang X, Zhang X, Duan E.
Stem Cell Rev. 2014 May 4. (Epub ahead of print)
Rotating microgravity-bioreactor cultivation enhances hepatic differentiation of mouse ESCs
Wang Y, Zhang Y, Zhang S, Peng G, Liu T, Li Y, Xiang D, Wassler MJ, Shelat HS, Geng Y
Tissue Eng Part A. 2012 Nov
Scaffold-free and scaffold-assisted 3D culture enhances differentiation of bone marrow stromal cells.
Jung Y, Ji H, Chen Z, Fai Chan H, Atchison L, Klitzman B, Truskey G, Leong KW
Sci Rep. 2015 Oct 12;5:15116. doi: 10.1038/srep15116
Scalable production of embryoid bodies with the rotary cell culture system
Wang XL, Wang CY, Yu XJ, Zhao YS, Li J, Duan CM, Guo XM
Sheng Li Xue Bao. 57:486-492, 2005
Slow turning lateral vessel bioreactor improves embryoid body formation and cardiogenic differentiation of mouse embryonic stem cells
Rungarunlert S, Klincumhom N, Tharasanit T, Techakumphu M, Pirity MK, Dinnyes A
Cell Reprogram. 2013 Oct;15(5):443-58. doi: 10.1089/cell.2012.0082
Spheriod cultures promote the stemness of corneal stromal cells
Li H1, Dai Y2, Shu J3, Yu R4, Guo Y2, Chen J5
Tissue Cell. 2014 Nov 7. pii: S0040-8166(14)00116-5. doi: 10.1016/j.tice.2014.10.008 [Epub ahead of print]
The combination of three dimensional and rotary cell culture system promotes the proliferation and maintains the differentiation potential of rat BMSCs
Tang Y, Xu Y, Xiao Z, Zhao Y, Li J, Han S, Chen L, Dai B, Wang L, Bing Chen B & Wang H
Scientific Reports 2017; 7:192
The effect of Smads signal pathway on the osteogenesis of human periodontal ligament stem cells
Li Y, Li S, Niu ZY, Bao B, Shi X
Shanghai Kou Qiang Yi Xue. 2012 Jun
The effect of the microgravity rotating culture system on the chondrogenic differentiation
Wu X, Li SH, Lou LM, Chen ZR
Mol Biotechnol. 2013 Jun
The effects of spheroid formation of adipose-derived stem cells in a microgravity bioreactor on stemness properties and therapeutic potential.
Zhang S, Liu P, Chen L, Wang Y, Wang Z, Zhang B
Biomaterials. 2015 Feb;41:15-25. doi: 10.1016/j.biomaterials.2014.11.019. Epub 2014 Dec 1.
The impact of simulated and real microgravity on bone cells and mesenchymal stem cells
Ulbrich C, Wehland M, Pietsch J, Aleshcheva G, Wise P, van Loon J, Magnusson N, Infanger M, Grosse J, Eilles C, Sundaresan A, Grimm D
Biomed Res Int. 2014;2014:928507. doi: 10.1155/2014/928507.
The simulated microgravity enhances multipotential differentiation capacity of bone marrow MSCs
Wang N, Wang H, Chen J, Zhang X, Xie J, Li Z, Ma J, Wang W, Wang Z
Cytotechnology. 2014 Jan
The use of murine embryonic stem cells, alginate encapsulation, and rotary microgravity bioreactor in bone tissue engineering
Hwang YS, Cho J, Tay F, Heng JY, Ho R, Kazarian SG, Williams DR, Boccaccini AR, Polak JM, Mantalaris A
Biomaterials 30: 499-507, 2008
Three-dimensional simulated microgravity culture improves the proliferation and odontogenic differentiation of dental pulp stem cell in PLGA scaffolds implanted in mice.
Li Y, He L, Pan S, Zhang L, Zhang W, Yi H, Niu Y.
Mol Med Rep. 2017 Feb;15(2):873-878. doi: 10.3892/mmr.2016.6042. Epub 2016 Dec 14.
Tissue-Engineered Nerve Constructs Under a Microgravity System for Peripheral Nerve Regeneration
Luo H, Zhu B, Zhang Y, Jin Y
Tissue Eng Part A. 2014 Sep 16. (Epub ahead of print)
Transfection of the IHH gene into rabbit BMSCs in a simulated microgravity environment promotes chondrogenic differentiation and inhibits cartilage aging.
Liu PC, Liu K1, Liu JF, Xia K, Chen LY, Wu X
Oncotarget. 2016 Sep 6;7(39):62873-62885. doi: 10.18632/oncotarget.11871.
U94 of human herpesvirus 6 down-modulates Src, promotes a partial mesenchymal-to-epithelial transition and inhibits tumor cell growth, invasion and metastasis.
Caccuri F, Ronca R, Laimbacher AS, Berenzi A, Steimberg N, Campilongo F, Mazzuca P, Giacomini A, Mazzoleni G, Benetti A, Caselli E, Presta M, Di Luca D, Fraefel C, Caruso A.
Oncotarget. 2017 May 11. doi: 10.18632/oncotarget.17817.
Uniform Embryoid Body Production and Enhanced Mesendoderm Differentiation with Murine Embryonic Stem Cells in a Rotary Suspension Bioreactor.
Lei X, Deng Z, Duan E
Methods Mol Biol. 2016 Apr 27.
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