Synthecon: 3D Culture Systems for 3D World!

Research Publications / Liver/ Pancreas


Simulated microgravity promotes the formation of tridimensional cultures and stimulates pluripotency and a glycolytic metabolism in human hepatic and biliary tree stem/progenitor cells. Costantini D, Overi D, Casadei L, Cardinale V, Nevi L, Carpino G, Di Matteo S, Safarikia S, Valerio M, Melandro F, Bizzarri M, Manetti C, Berloco PB, Gaudio E, Alvaro D. Sci Rep. 2019; 9(1):5559. [Full Text]

Rotational culture and integration with amniotic stem cells reduce porcine islet immunoreactivity in vitro and slow xenorejection in a murine model of islet transplantation.  Zafar A, Lee J,  Yesmin S, Paget MB, Bailey CJ, Murray, HE, Downing R. Xenotransplantation 2019; e12508  [Abstract]

Mesenchymal stem cells support growth and organization of host-liver colorectal-tumor organoids and possibly resistance to chemotherapy. Devarasetty M, Wang E, Soker S, Skardal A. Biofabrication. 2017;9(2)5090 [Abstract] 
Direct orthotopic implantation of hepatic organoids.

Zhou VX, Lolas M, Chang TT.J Surg Res. 2017;211:251-260. [Abstract]

Hetero-cellular prototyping by synchronized multi-material bioprinting for rotary cell culture system.
Snyder J1, Son AR, Hamid Q, Wu H, Sun W. Biofabrication. 2016 Jan 13;8(1):015002. [Abstract]

Microgravity as a means to incorporate HepG2 aggregates in polysaccharide-protein hybrid scaffold.
Sarika PR, James NR, Anilkumar PR, Raj DK, Kumary TV. J Mater Sci Mater Med. 2016 Feb;27(2):27. doi: 10.1007/s10856-015-5638-5. [Abstract]

Liver-Tumor Hybrid Organoids for Modeling Tumor Growth and Drug Response In Vitro.
Skardal A, Devarasetty M, Rodman C, Atala A, Soker S. AnnBiomed Eng. 2015 Mar 17.doi: 10.1007/s10439-015-1298-3 [Abstract]

Low gravity rotational culture and the integration of immunomodulatory stem cells reduce human islet allo-reactivity. 
Qureshi KM, Lee J, Paget MB, Bailey CJ, Curnow SJ, Murray HE, Downing R. Clin. Transplant. 2015 Jan;29(1):90:8. doi: 10.1111/ctr.12488. Epub 2014 Dec 5. [Abstract] [Full Text pdf]

Screening candidate metastasis-associated genes in three-dimensional HCC spheroids with different metastasis potential.
Chen R, Dong Y, Xie X, Chen J, Gao D, Liu Y, Ren Z, Cui J. Int J Clin Exp Pathol. 2014 Apr 15;7(5):2527-35. eCollection 2014. [Abstract] [Full Text] [Full Text pdf]
Dynamic expression patterns of differential proteins during early invasion of hepatocellular carcinoma.
Chen RX, Song HY, Dong YY, Hu C, Zheng QD, Xue TC, Liu XH, Zhang Y, Chen J, Ren ZG, Liu YK, Cui JF. PLoS One. 2014 Mar 10;9(3):e88543. doi: 10.1371/journal.pone.0088543. eCollection 2014.[Abstract] [Full Text] [Full Text pdf]
Molecular mechanisms underlying the enhanced functions of three-dimensional hepatocyte aggregates.
Chang TT, Hughes-Fulford M.; Biomaterials. 2014 Feb;35(7):2162-71.  doi:10.1016/j.biomaterials.2013.11.063. [Abstract]
Rotational Transport of Islets: The Best Way for Islets to Get around?
Oberhuber R, Mittermair C, Zelger B, Pirkebner D, Draxl A, Weissenbacher A, Resch T, Margreiter C, Sucher R, Margreiter R, Pratschke J, Hengster P, Hermann M.; Biomed Res Int. 2013;2013:975608. doi: 10.1155/2013/975608.[Abstract] [Full Text] [Full Text pdf]
Antiproliferation activity of Devil's club (Oplopanax horridus) and anticancer agents on human pancreatic cancer multicellular spheroids.
Tai J, Cheung SS, Ou D, Warnock GL, Hasman D.; Phytomedicine. 2013 Nov 8. pii: S0944-7113(13)00401-7. doi: 10.1016/j.phymed.2013.10.003. [Abstract]
Simulated microgravity combined with polyglycolic Acid scaffold culture conditions improves the function of pancreatic islets.Song Y, Wei Z, Song C, Xie S, Feng J, Fan J, Zhang Z, Shi Y. Biomed Res Int. 2013;2013:150739. doi: 10.1155/2013/150739. [Abstract] [Full Text] [Full Text pdf]
Replication of hepatitis E virus in three-dimensional cell culture.
Berto A, Van der Poel WH, Hakze-van der Honing R, Martelli F, La Rogione RM, Inglese N, Collins J, Grierson S, Johne R, Reetz J, Dastjerdi A, Banks M. J Virol Methods. 2013 Feb;187(2):327-32. doi: 10.1016/j.jviromet2012.10.017.Epub 2012 Nov 14 [Abstract] [Full Text]
Rotating microgravity-bioreactor cultivation enhances the hepatic differentiation of mouse embryonic stem cells on biodegradable polymer scaffolds.Wang Y, Zhang Y, Zhang S, Peng G, Liu T, Li Y, Xiang D, Wassler MJ, Shelat HS, Geng Y. Tissue Eng Part A. 2012 Nov;18(21-22):2376-85. doi: 10.1089/ten.TEA.2012.0097. 2012 Sep 24. [Abstract] 
Reconstitution of hepatic tissue architectures from fetal liver cells obtained from a three-dimensional culture with a rotating wall vessel bioreactor.
Ishikawa M, Sekine K, Okamura A, Zheng YW, Ueno Y, Koike N, Tanaka J, Taniguchi H. J Biosci Bioeng.  June 2011, 111(6):711-8. [Abstract]
Labeling of primary human hepatocytes with micron-sized iron oxide particles in suspension culture suitable for large-scale preparation.
Kammer NN, Billecke N, Morgul MH, Adonopoulou MK, Mogl M, Huang MD, Florek S, Schmitt KR, Raschzok N, Sauer IM. Artif Organs. 35(4):E91-100. doi: 10.1111/j.1525-1594.2010.01177.x., 2011 [abstract]

Rotational co-culture of clonal β-cells with endothelial cells: effect of PPAR-γ agonism in vitro on insulin and VEGF secretion.
Paget MB1, Murray HE, Bailey CJ, Flatt PR, Downing R. Diabetes Obes Metab. 2011 Jul;13(7):662-8. doi: 10.1111/j.1463-1326.2011.01392.x. [Abstract] 
Low-shear modeled microgravity environment maintains morphology and differentiated functionality of primary porcine hepatocyte cultures.
Nelson LJ, Walker SW, Hayes PC, Plevris JN. Cells Tissues Organs 192:125-140, 2010 [Abstract]
Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection.
Sainz B Jr, TenCate V, Uprichard SL.Virol J 6:103, 2009 [Abstract] [Full Text] [Full Text pdf]
Transplantation of sertoli-islet cell aggregates formed by microgravity: prolonged survival in diabetic rats.
Han X, Qui L, Zhang Y, Kong Q, Wang H, Wang H, Li H, DUan C, Wang Y, Song Y, Wang C. Exp Biol Med (Maywood). 234:595-603, 2009 [Abstract] [Full Text] [Full Text pdf]
In-vitro reconstitution of hepatic tissue architecture with neonatat mouse liver cells using three –dimensional culture.
Okamura A, Zheng YW, Hirochika R, Tanaka J, Taniguchi H.; J Nanosci Nanotechnol 7: 721- 725, 2007 [Abstract]
Genome-wide gene expression profiling of microgravity effect on human liver cells.
Clement JQ, Lacy SM, Wilson BL. J Gravit Physiol 14: P121-122, 2007 [Abstract]
Immature syngeneic dendritic cells potentiate tolerance to pancreatic islet allografts depleted of donor dendritic cells in microgravity culture condition.
Stepkowski SM, Phan T, Zhang H, Bilinski S, Kloc M, Qi Y, Katz SM, Rutzky LP. Transplantation 82:1756-1763, 2006 [Abstract]
Preservation of glucose responsiveness in human islets maintained in a rotatinal cell culture system.
Murray H.E., Padget, M.B, Downing, R.: Molecular and Cellular Endocrinology 238: 39-49, 2005 [Abstract]
Proliferation Rates of HepG2 Cells Encapsulated in Alginate Are Increased in a Microgravity Environment Compared With Static Cultures.
Coward SM, Selden C, Mantalaris A, Hodgson HJ: Artif Organs 29: 152-158, 2005. [Abstract]
Experimental Study on Islet Cells in Rats Under Condition of Three-dimensional Microgravity.
Song C, Duan XQ, Li X, Han LO, Xu P, Song CF, Jin LH: Zhonghua Wai Ke Za Zhi 42: 559-561, 2004. [Abstract in English, Article in Chinese]
Selection of Scaffolds of Rat Hepatocytes in Three- dimensional Culture Under Simulated Microgravity.
Zhang SQ, Gao SJ, Jiang QY, Lao QL, Feng DY: Shi Yan Sheng Wu Xue Bao 37: 67-71, 2004. [Abstract in English]
Experimental Study of Rat Beta Islet Cells Cultured under Simulated Microgravity Conditions.
Song C, Duan XQ, Li X, Han LO, Xu P, Song CF, Jin LH: Acta Biochim Biophys (Shanghai) 36: 47-50, 2004. [Abstract] [Full Text]
Microgravity Culture Conditions Reduces Immunogenicity And Improves Function Of Pancreatic Islets.
Rutzky L, Bilinzki Z, Kloc M, Phan T, Zhang H, Katz S, Stepkowski S: Transplantation 74: 13-21, 2002 [Abstract]
Maintenance of Liver Functions in Rat Hepatocytes Cultured as Spheroids in a Rotating Wall Vessel.
Brown LA, Arterburn LM, Miller AP, Cowger NL, Hartley SM, Andrews A, Silber PM, Li AP:In Vitro Cell Dev Biol Anim Jan; 39: 13-20,2003. [Abstract]
A Unique Cytoplasmic Marker for Extratesticular Sertoli Cells.
Cameron DF, Hushen JJ, Dejarlais T, Colado L, Wolski KM, Sanberg PR, Saporta S:Cell Transplant 11: 507-512, 2002. [Abstract]
Formation of Insulin-Secreting, Sertoli-Enriched Tissue Constructs by Microgravity Cocultures of Isolated Pig Islets and Rat Sertoli cells.
Cameron DF, Hushen JJ, and Nazian SJ,In Vitro Cell Dev Biol Anim 37:490-498, 2001. [Abstract]
Altered TNF-Alpha, Glucose, Insulin, and Amino Acids in Islets of Langerhans Cultured in a Microgravity Model System.
Tobin BW, Leeper-Woodfored SK, Hashemi BB, Smith SM, and Sams CF,Am J Physiol Endocrinol Metab 280:E92-102, 2001[Abstract] [Full Text] [Full Text pdf]
Microarray Analysis of Genes Differentially Expressed in HEPG2 Cells Cultured in Simulated Microgravity: Preliminary Report.
Khaoustov VI, Risin D, Pellis NR, Yoffe B.: In Vitro Cell Dev Biol Anim 37: 84-8, 2001. [Abstract]
Microgravity Culture Conditions Decrease Immunogenicity but Maintain Excellent Morphology of Pancreatic Islets.Rutzky L, Kloc M, Bilinski S, Phan T, Zhang H, Stepkowski SM, Katz S.:  Transplant Proc 33: 388, 2001. [Abstract]
The Simulated Microgravity Environment Maintains Key Metabolic Functions and Promotes Aggregation of Primary Porcine Hepatocytes.Dabos KJ, Nelson LJ, Bradnock TJ, Parkinson JA, Sadler IH, Hayes PC, Plevris JN:  Biochem Biophys Acta 1526: 119-130, 2001. [Abstract]
Cultures of Human Liver Cells in Simulated Microgravity Environment.
Yoffe B, Darlington GJ, Soriano HE, Krishman B, Risin D, Pellis NR, Khaustov VI:Adv Space Res 24: 829-836, 1999. [Abstract]
Rotary Cell Culture System (RCCS): A New Method for Cultivation Hepatocytes on Microcarriers.
Mitteregger R, Vogt G, Rossmanith E, Falkenhagen D.,  Int J Artif Organs 22: 12 816-12822, 1999. [Abstract]
Effects of Microgravity on the Embryonic Pancreas.
Rose MI, Brown DC, Pellis NR, Crisera CA, Colen KL, Longaker MT, Gottes GK:In Vitro Cell Dev Biol Anim 35: 560-563, 1999 [Abstract]
Progressive Maturation Resistance to Microcystin-LR Cytotoxicity in Two Different Hepatospheroidal Models.
Battle T, Maguire T, Moulsdale H, Doyle A. Cell Biol Toxicol 15: 3-12, 1999. [Abstract]
Induction of Three- Dimensional Assembly of Human Liver Cells by Simulated Microgravity.
Khaoustov VI, Darlington GJ, Soriano HE, Krishnan B, Risen D, Pellis NR, Yoffe B: In Vitro Cell Dev Biol Animal, 35: 501-509. 1999. [Abstract]
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